Sapna Deo
Research
Our research work is at the interface of analytical chemistry and
biological chemistry with the goal of developing novel bioanalytical
techniques employing luminescent and fluorescent proteins. Specifically,
we are working on development of sensing systems for different classes of
RNAs, including microRNAs, mRNAs, and siRNAs. We capitalize on high sensitivity
of detection associated with luminescent proteins and combine it with one step
detection methods such as protein reassembly and bioluminescence resonance
energy transfer in order to develop highly sensitive as well as fast detection
methods for nucleic acids.
A variety of fluorescent proteins with unique properties have been characterized.
For example, fluorescent proteins that display different color emission of light are
available. Our research focuses on genetic modification of fluorescent proteins followed
by their applications in the development of single-step highly sensitive assays. The
biosenseing systems for the detection of biomarkers of disease, high throughput screening
and for studying protein-protein interactions will be developed. Application of the biosensing
systems in metabonomics, diagnostics, biomedical research, and environmental analysis is
envisioned.
Another area of our research interest is the development of methods for
analysis of intrinsically disordered proteins and their interactions with
target ligands, proteins, and DNA. Unstructured regions are common in
cancer-associated an signal-transduction proteins These unstructured segments
in protein undergo coupled folding and binding. The focus of our work is the design
and development of luminescence-based "molecular switches" for quantitative in vitro
and in vivo analysis of disordered protein-target interactions.
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Recent Publications
K. A. Cissel, Y. Rahimi, S. Shrestha, E. A. Hunt, L. Armes, A. Ault and S. K. Deo
"Bioluminescent detection of miRNA, miR21 in breast cancer cells" Analytical Chemistry
2008, 80, (Accelerated Article), in press.
A. M. Goulding, S. Shrestha, E. A. Hunt, K. Dria and S. K. Deo "Biochemical and
spectroscopic characterization of non-natural variants of DsRed-monomer" Protein
Engineering Design & Selection
2008, in press.
L. A. Rowe, A. Rothert, C. Logue, C. M. Ensor, S. K. Deo and S. Daunert "Spectral tuning
of Ca-Regulated photoproteins using site- directed mutagenesis and chromophore analogues"
Protein Engineering Design & Selection
2008, in press.
K. A. Cissel, S. Shrestha, J. L. Purdie, D. Kroodsma and S. K. Deo "Development of a
molecular biosensing system based on binding- induced conformational changes observed in
intrinsically disordered proteins" Analytical & Bioanalytical Chemistry
2008, available
online.
Y. Rahimi, S. Shrestha, T. Banerjee and S. K. Deo "Copper sensing system based on the
far-red fluorescent protein, HcRed from Heteractis crispa" Analytical Biochemistry
2007,
370, 60-67.
S. K. Deo, K. A. Cissel, A. Goulding, Y. Rahimi and S. Shrestha "Biochemistry,
structure, and engineering of red fluorescent proteins", Chapter 6, in: Luciferases and
Fluorescent Proteins Technology: Principles and Advances in Biotechnology and
Bioimaging, V. R. Viviani and Y. Ohmiya, Eds., Research Signpost Press,
2007.
K. A. Cissel, S. Shrestha and S. K. Deo "microRNA detection: Challenge for analytical
chemists" Analytical Chemistry
2007, 79, 4754-4761.
Y. Rahimi, S. Shrestha and S. K. Deo "Metal affinity-based purification of a red
fluorescent protein" Chromatographia
2007, 65, 429-433.
L. A. Doleman, L. Davis, L. Rowe, E. A. Moschou, S. K. Deo and S. Daunert
"Bioluminescence DNA hybridization assay for Plasmodium falciparum based on the
photoprotein aequorin" Analytical Chemistry
2007, 79, 4149 -4153.
L. A Rowe, S. K. Deo, J. Shofner, M. Ensor, S. Daunert and S. K. Deo "Aequorin-based
homogeneous cortisol immunoassay for analysis of saliva samples" Bioconjugate Chemistry
2007, 18, 1772-1777.
K. Turner, S. Xu, P. Pasini, S. K. Deo and S. Daunert "Hydroxylated polychlorinated
biphenyls detection based on a genetically engineered bioluminescent whole-cell sensing
system" Analytical Chemistry
2007, 79, 5740-5745.
S. Daunert, L. G. Bachas, K. Gregory, S. K. Deo, V. Vukasinovic and G. Shrift
"Calmodulin mediated reversible immobilization of enzymes" Colloids and Sufaces:
Biointerfaces
2007,58, 20-27.
S. Shrestha, S. K. Deo, "Bioluminescence Resonance Energy Transfer in
Bioanalysis", Chapter 6, in: Photoproteins in
Bioanalysis, Wiley-VCH, 2006, pp. 95-111.
E. C. Dikici, L. Rowe, A. Rothert, S. K. Deo, S. Daunert
"Photoprotein in miniaturized microfluidics Systems" Chapter 10, in:
Photoproteins in Bioanalysis, Wiley-VCH,
2006, pp. 179-198.
J. S. Feliciano, P. Pasini, S. K. Deo, S. Daunert "Photoprotein as
reporters in whole cell sensing" Chapter 8, in: Photoproteins
in Bioanalysis, Wiley-VCH, 2006, pp. 131-154.
L. A. Doleman, S. Bachas-Daunert, L. Davis, S. Daunert , S. K. Deo
"Photoproteins and Instrumentation: Their availability and
applications in bioanalysis", Chapter 12, in: Photoproteins in
Bioanalysis, Wiley-VCH, 2006, pp. 225-234.
S. K. Deo "High Throughput
Screening in Drug Discovery" Analytical & Bioanalytical
Chemistry 2006, 385, 661-662.
S. Shreshta, S. K. Deo, "Anthozoa Red Fluorescent Protein in
Biosensing" Analytical & Bioanalytical Chemistry
2006, 386, 515-524.
S. K. Deo, S. Daunert "Quo vadis" Leading the way with the younger
generation of scientists" Analytical & Bioanalytical
Chemistry 2006, 386, 401-402.
E. A. Moschou, L. G. Bachas, S. Daunert, S. K. Deo, "Hinge-motion
binding protein: Unraveling their analytical potential"
Analytical Chemistry (News & Features)
2006, 78, 6693-6700.
A. Kumari, P. Pasini, S. K. Deo, D. Flomenhoft,
H. Shashidhar, S. Daunert, "Biosensing Systems for the Detection of
Bacterial Quorum Signaling Molecules" Analytical
Chemistry 2006,
78, 7603 -7609.
Y. Rahimi, S. Shrestha, T. Banerjee, S. K. Deo "Study of Metal Binding
to DsRed-Monomer" Proceedings of the International Society of
Chemiluminescence and Bioluminescence, 2006.
S. Xu, E. D'Angelo, D. Ghosh, J.
Feliciano, S. K. Deo, and S. Daunert, "Detection of Polychlorinated
Biphenyls Employing Chemical Dechlorination Followed by a Biphenyl
Whole Cell Sensing System" Toxicol. Environ. Chem.
2006, 87, 287-298.
S. Daunert, L. G. Bachas, K. Gregory, S. K. Deo, V. Vukasinovic, G.
Shrift "Calmodulin mediated reversible immobilization of enzymes"
Colloids and Surfaces Biointerfaces,
2006 (published online October 27, 2006).
S. K. Deo, M. Mirasoli, S. Daunert "Bioluminescence resonance
energy transfer from aequorin to a fluorophore: an artificial
jellyfish for applications in multianalyte detection"
Analytical & Bioanalytical Chemistry
2005, 381, 1387-1394.
J. D. Ehrick, S. K. Deo, T. W. Browning, L.G. Bachas, M. J. Madou, S.
Daunert, "Stimuli-sensitive hydrogels with tailored response
characteristics: integration of genetically engineered proteins within
synthetic networks" Nature Materials
2005, 4, 298-302.
Rothert, S. K. Deo, R. S. Shetty, L. Puckett,, L. Millner, M. J.
Madou, S. Daunert "Whole cell based assay for antimonite and arsenite
on a compact disc microfluidic platform" Analytical
Biochemistry 2005, 342, 11-19.
B. V. Sharma, S. Shrestha, S. K. Deo and S. Daunert "Sensors based on
periplasmic binding proteins" Chapter 3 in: Fluorescence
Sensors and Biosensors, R. Thompson, Ed., 2005,
pp. 45-67.
B. V. Sharma, S. K. Deo, L. G. Bachas, and S. Daunert "Rapid,
competitive FRET assay for the detection of calmodulin antagonists"
Bioconjugate Chemistry 2005,
16, 1257-1263.
Publications Prior to 2005
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